منابع مشابه
Controlled damage in thick specimens by multiphoton excitation.
Controlled damage by light energy has been a valuable tool in studies of cell function. Here, we show that the Ti:Sapphire laser in a multiphoton microscope can be used to cause localized damage within unlabeled cells or tissues at greater depths than previously possible. We show that the damage is due to a multiphoton process and made wounds as small as 1 microm in diameter 20 microm from the ...
متن کاملSystem for combined three-dimensional morphological and molecular analysis of thick tissue specimens.
We present a new system for simultaneous morphological and molecular analysis of thick tissue samples. The system is composed of a computer-assisted microscope and a JAVA-based image display, analysis, and visualization program that allows acquisition, annotation, meaningful storage, three-dimensional reconstruction, and analysis of structures of interest in thick sectioned tissue specimens. We...
متن کاملOptimal clearing and mounting media for confocal microscopy of thick specimens
Sample preparation for confocal microscopy typically involves fixing and staining, followed by clearing and mounting. Clearing involves treatment with solvent to dissolve lipids and other structures that cause light scattering and reduce the ability to image through thick specimens. Mounting involves transfer of the specimen into a medium which matches the refractive index the objective is desi...
متن کاملDark-field X-ray ptychography: Towards high-resolution imaging of thick and unstained biological specimens
The phase shift of light or electrons in objects is now necessary for probing weak-phase objects such as unstained biological specimens. Optical microscopy (OM) and transmission electron microscopy (TEM) have been used to observe weak-phase objects. However, conventional OM has low spatial resolution and TEM is limited to thin specimens. Here, we report on the development of dark-field X-ray pt...
متن کاملPractical image restoration of thick biological specimens using multiple focus levels in transmission electron microscopy.
Three-dimensional electron tomographic studies of thick specimens such as cellular organelles or supramolecular structures require accurate interpretations of transmission electron micrograph intensities. In addition to microscope lens aberrations, thick specimen imaging is complicated by additional distortions resulting from multiple elastic and inelastic scattering. Extensive analysis of the ...
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ژورنال
عنوان ژورنال: Botanical Gazette
سال: 1886
ISSN: 0006-8071
DOI: 10.1086/325961